特超敏ECL發光底物檢測試劑盒用于檢測直接或間接標記辣根過氧化物酶HRP的抗體及其關聯的抗原，是一種可廣泛應用于免疫學實驗的化學發光試劑。由于采用了獨特的發光底物系統和高靈敏度熒光ECL發光試劑，特超敏ECL發光底物檢測試劑盒是目前靈敏的商業化熒光ECL檢測試劑之一，靈敏度高于常規超敏ECL檢測試劑數十倍乃至數百倍：
1、具有極高靈敏度和高信噪比，可檢測10～100fg微量抗原；
2、發光迅速且穩定，熒光可使X光膠片感光達12小時以上，特別適用于痕量蛋白或核酸檢測；
3、可使用更高的抗體稀釋倍數(1:5000～1:20000)，極其節省抗體。
4、優異的性價比，更高的性能，更低的價格。
1、本產品僅限于專業人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內。
2、為了您的安全和健康，請穿實驗服并戴一次性手套操作，且戴手套可以避免在印記膜上留下手印。
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答：可能的原因有：
1、目的蛋白有多個修飾位點，本身可以呈現多條帶，建議查閱文獻或進行生物信息學分析，獲得蛋白序列的修飾位點信息，通過去修飾確定蛋白實際大小；
2、樣本處理過程中目的蛋白發生降解,建議加入蛋白酶抑制劑；樣本處理時在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強，建議使用特異性強的抗體；
5、抗體孵育時間過久，建議減少抗體孵育時間；
6、二抗與抗原有交叉反應，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質變性過程及強度；
8、底物顯色與曝光時間過長，建議縮短顯色及曝光的時間。
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