WB, IHC
Goat
N/A
IgG1
1:200-1:2,000 for immunohisto/cytochemistry
1:3,000-1:80,000 for ELLSA and Western blotting with chromogenic substrates
1:5,000-1:100,000 for Western blotting with ECL substates
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to
agarose beads.
Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule goat IgG.
lt also reacts with the light chains of other goat immunoglobulins. No antibody was detected against
non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other
species.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results
are acceptable for the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
1.Ying T ,Yongyong Y ,Yue W , et al.Developmental exposure to chlorpyrifos causes neuroinflammation via necroptosis in mouse hippocampus and human microglial cell line.[J].Environmental pollution (Barking, Essex : 1987),2022,314120217-120217.(IF 9.988)
答：可能的原因有：
1、目的蛋白有多個修飾位點，本身可以呈現多條帶，建議查閱文獻或進行生物信息學分析，獲得蛋白序列的修飾位點信息，通過去修飾確定蛋白實際大小；
2、樣本處理過程中目的蛋白發生降解,建議加入蛋白酶抑制劑；樣本處理時在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強，建議使用特異性強的抗體；
5、抗體孵育時間過久，建議減少抗體孵育時間；
6、二抗與抗原有交叉反應，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質變性過程及強度；
8、底物顯色與曝光時間過長，建議縮短顯色及曝光的時間。
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