PBS, pH 7.4, 15mg/ml Bovine Serum Albumin as Stablizer and 50% Glycerol.Store at -20°C. Do not aliquot the antibody.
1:500-1:5,000 for immunohisto/cytochemistry
1:5,000-1:100,000 for ELISA and Western blotting with chromogenic
substrates 1:10,000-1:200,000 for Western blotting with ECL substates
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled
to agarose beads.
Based on immunoelectrophoresis and/or ELISA,the antibody reacts with whole molecule rabbit IgG. lt also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
The antibody may cross-react with immunoglobulins from other species.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable for the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
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答：可能的原因有：
1、目的蛋白有多個修飾位點，本身可以呈現多條帶，建議查閱文獻或進行生物信息學分析，獲得蛋白序列的修飾位點信息，通過去修飾確定蛋白實際大小；
2、樣本處理過程中目的蛋白發生降解,建議加入蛋白酶抑制劑；樣本處理時在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強，建議使用特異性強的抗體；
5、抗體孵育時間過久，建議減少抗體孵育時間；
6、二抗與抗原有交叉反應，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質變性過程及強度；
8、底物顯色與曝光時間過長，建議縮短顯色及曝光的時間。
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