IF
Chicken
150KD
IgG1
0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6 Store at -20°C.
Do not aliquot the antibody.
2mg/ml
Goat Polyclonal IgG, affinity purified by Protein A.
IF=1:100-1000
Cy3 bis-NHS ester Amax=514nm,552 nm; Emax=570 nm
The antibody was purified from antisera by immunoaffinity chromatographyusing antigens coupled to
agarose beads.
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17
mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are
produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two
separate functions; to bind to the pathogen that elicited the response and to recruit other cells and
molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination
and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results
are acceptable for the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
更新中...
答：可能的原因有：
1、目的蛋白有多個修飾位點，本身可以呈現(xiàn)多條帶，建議查閱文獻或進行生物信息學分析，獲得蛋白序列的修飾位點信息，通過去修飾確定蛋白實際大小；
2、樣本處理過程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑；樣本處理時在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強，建議使用特異性強的抗體；
5、抗體孵育時間過久，建議減少抗體孵育時間；
6、二抗與抗原有交叉反應，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質變性過程及強度；
8、底物顯色與曝光時間過長，建議縮短顯色及曝光的時間。
Copyright ?湖北普美生物科技有限公司 All Rights Reserved. 工信部備案：鄂ICP備17001029號-1 技術支持：武漢微盟
