WB, IP, IF
N/A
N/A
IgG1
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
WB: 1:5,000
IP: 1:200
IF: 1:1000
The HA tag antibody can recognize C-terminal, internal, and N-terminal HA-tag fusion proteins.
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus.
The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used
as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express
the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant
protein.HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion
protein detection.
更新中...
答：可能的原因有：
1、目的蛋白有多個修飾位點，本身可以呈現多條帶，建議查閱文獻或進行生物信息學分析，獲得蛋白序列的修飾位點信息，通過去修飾確定蛋白實際大小；
2、樣本處理過程中目的蛋白發生降解,建議加入蛋白酶抑制劑；樣本處理時在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強，建議使用特異性強的抗體；
5、抗體孵育時間過久，建議減少抗體孵育時間；
6、二抗與抗原有交叉反應，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質變性過程及強度；
8、底物顯色與曝光時間過長，建議縮短顯色及曝光的時間。
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