WB, IHC
H, R, M, Mk, Dg, C, Hm, Rb, Sh
55KD
IgG
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C.
Do not aliquot the antibody.
WB: 1:2000-5,000 IHC: 1:200
The β- tubulin Rabbit Polyclonal Antibody can detects endogenous β- tubulin protein.
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton.
They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies
against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that
levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue
is very low and therefore β-Tubulin should not be used as loading control for these tissues.
1.Xiaoyu D ,Yiting F ,Dongqin X , et al.Targeting macrophagic 17β-HSD7 by fenretinide for the treatment of nonalcoholic fatty liver disease[J].Acta Pharmaceutica Sinica B,2023,13(1):142-156. (IF 14.903)
答：可能的原因有：
1、目的蛋白有多個(gè)修飾位點(diǎn)，本身可以呈現(xiàn)多條帶，建議查閱文獻(xiàn)或進(jìn)行生物信息學(xué)分析，獲得蛋白序列的修飾位點(diǎn)信息，通過去修飾確定蛋白實(shí)際大小；
2、樣本處理過程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑；樣本處理時(shí)在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強(qiáng)，建議使用特異性強(qiáng)的抗體；
5、抗體孵育時(shí)間過久，建議減少抗體孵育時(shí)間；
6、二抗與抗原有交叉反應(yīng)，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質(zhì)變性過程及強(qiáng)度；
8、底物顯色與曝光時(shí)間過長(zhǎng)，建議縮短顯色及曝光的時(shí)間。
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