Flow Cyt,ICC/IF,IHC
Mouse
N/A
IgG1
0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6 Store at -20°C.
Do not aliquot the antibody.
15mg/ml Bovine Serum Albumin (IgG-Free,Protease-Free)
0.05% Sodium Azide
1:100-1:800 for most applications
Fluorescein-5-isothiocyanate(FITC)Amax=492nm;Emax=520nm
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to
agarose beads.
Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule mouse IgG. lt also
reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-
immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are
acceptable for the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
1.Tingting L ,Xiazhou F ,Yaoli L , et al.Sulforaphane Inhibits Osteoclastogenesis via Suppression of the Autophagic Pathway[J].Molecules,2021,26(2):347-347. (IF 4.412)
2.Sun Y ,Chen Y ,Zhao H , et al.Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma[J].iScience,2024,27(4):109340-. (IF 4.6)
3.HuiJian C ,XiYue Y ,Ao S , et al.High-Fat-Diet-Induced Extracellular Matrix Deposition Regulates Integrin - FAK Signals in Adipose Tissue to Promote Obesity.[J].Molecular nutrition & food research,2022,66(7):e2101088-e2101088. (IF 6.575)
4.Wangming Z ,Xiaojing Y ,Linchun B , et al.Bisbenzylisoquinoline alkaloid fangchinoline derivative HY-2 inhibits breast cancer cells by suppressing BLM DNA helicase.[J].Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie,2023,169115908-115908. (IF 7.5)
5.Yongxi D ,Jun L ,Shanhui Z , et al.Design, synthesis and bioevaluation of 1,2,4-thiadiazolidine-3,5-dione derivatives as potential GSK-3β inhibitors for the treatment of Alzheimer's disease[J].Bioorganic Chemistry,2023,134106446-106446. (IF 5.1)
答：可能的原因有：
1、目的蛋白有多個修飾位點，本身可以呈現多條帶，建議查閱文獻或進行生物信息學分析，獲得蛋白序列的修飾位點信息，通過去修飾確定蛋白實際大小；
2、樣本處理過程中目的蛋白發生降解,建議加入蛋白酶抑制劑；樣本處理時在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強，建議使用特異性強的抗體；
5、抗體孵育時間過久，建議減少抗體孵育時間；
6、二抗與抗原有交叉反應，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質變性過程及強度；
8、底物顯色與曝光時間過長，建議縮短顯色及曝光的時間。
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