Flow Cyt,ICC/IF,IHC
Rabbit
N/A
IgG1
0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6 Store at -20°C.
Do not aliquot the antibody.
15mg/ml Bovine Serum Albumin (IgG-Free,Protease-Free)
0.05% Sodium Azide
1:50-1:200 for most applications
Fluorescein-5-isothiocyanate(FITC)Amax=492nm;Emax=520nm
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to
agarose beads.
Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule rabbit IgG. lt also
reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-
immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are
acceptable for the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
更新中...
答：可能的原因有：
1、目的蛋白有多個(gè)修飾位點(diǎn)，本身可以呈現(xiàn)多條帶，建議查閱文獻(xiàn)或進(jìn)行生物信息學(xué)分析，獲得蛋白序列的修飾位點(diǎn)信息，通過(guò)去修飾確定蛋白實(shí)際大小；
2、樣本處理過(guò)程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑；樣本處理時(shí)在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強(qiáng)，建議使用特異性強(qiáng)的抗體；
5、抗體孵育時(shí)間過(guò)久，建議減少抗體孵育時(shí)間；
6、二抗與抗原有交叉反應(yīng)，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質(zhì)變性過(guò)程及強(qiáng)度；
8、底物顯色與曝光時(shí)間過(guò)長(zhǎng)，建議縮短顯色及曝光的時(shí)間。
Copyright ?湖北普美生物科技有限公司 All Rights Reserved. 工信部備案：鄂ICP備17001029號(hào)-1 技術(shù)支持：武漢微盟
