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Goat anti-Rat IgG (H+L), PE AffiniPure
貨號(hào):PMK-015-099M/S
一鍵復(fù)制產(chǎn)品信息
價(jià)格:
240.00
規(guī)格:
100ul
1ml
貨期:
現(xiàn)貨
本產(chǎn)品4℃運(yùn)輸,保存于-20℃,保質(zhì)期 12個(gè)月。
Details
產(chǎn)品詳情
  • Applications

    IF、Flow-Cyt

  • Species Cross-Reactivity

    Rat

  • Molecular Weight

    N/A

  • Isotype

    IgG1

  • Storage Buffer & Condition

    0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6 Store at -20°C.Do not aliquot the antibody.

  • Concentration

    2mg/ml

  • Preservative

    0.05% Sodium Azide

  • Also known as

    Immunoglobulin G

  • Application

    Flow-Cyt: 1:100-1000     IF:  1:100-1000 

    not yet tested in other applications.


  • Purity

    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

  • background

    mmunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. 

    IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our 

    adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response 

    and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic 

    recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.


  • Expiration date

    Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable 

    for the intended use.


  • Note

    For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.

Instruction manual/COA download
說明書/COA下載
Published literature
已發(fā)表文獻(xiàn)
  • 更新中...

Product Q&A FAQ
產(chǎn)品問答FAQ
  • Western Blot(WB)實(shí)驗(yàn)中有很多雜帶咋回事?

    答:可能的原因有:

    1、目的蛋白有多個(gè)修飾位點(diǎn),本身可以呈現(xiàn)多條帶,建議查閱文獻(xiàn)或進(jìn)行生物信息學(xué)分析,獲得蛋白序列的修飾位點(diǎn)信息,通過去修飾確定蛋白實(shí)際大小;

    2、樣本處理過程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑;樣本處理時(shí)在冰上操作;

    3、雜蛋白多,建議處理目的蛋白;

    4、抗體特異性不強(qiáng),建議使用特異性強(qiáng)的抗體;

    5、抗體孵育時(shí)間過久,建議減少抗體孵育時(shí)間;

    6、二抗與抗原有交叉反應(yīng),建議選擇合適的封閉物;

    7、二聚體或多聚體存在,建議增加蛋白質(zhì)變性過程及強(qiáng)度;

    8、底物顯色與曝光時(shí)間過長(zhǎng),建議縮短顯色及曝光的時(shí)間。


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