IF、Flow-Cyt
Rat
N/A
IgG1
0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6 Store at -20°C.Do not aliquot the antibody.
2mg/ml
0.05% Sodium Azide
Immunoglobulin G
Flow-Cyt: 1:100-1000 IF: 1:100-1000
not yet tested in other applications.
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
mmunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood.
IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our
adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response
and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic
recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable
for the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
更新中...
答:可能的原因有:
1、目的蛋白有多個(gè)修飾位點(diǎn),本身可以呈現(xiàn)多條帶,建議查閱文獻(xiàn)或進(jìn)行生物信息學(xué)分析,獲得蛋白序列的修飾位點(diǎn)信息,通過去修飾確定蛋白實(shí)際大小;
2、樣本處理過程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑;樣本處理時(shí)在冰上操作;
3、雜蛋白多,建議處理目的蛋白;
4、抗體特異性不強(qiáng),建議使用特異性強(qiáng)的抗體;
5、抗體孵育時(shí)間過久,建議減少抗體孵育時(shí)間;
6、二抗與抗原有交叉反應(yīng),建議選擇合適的封閉物;
7、二聚體或多聚體存在,建議增加蛋白質(zhì)變性過程及強(qiáng)度;
8、底物顯色與曝光時(shí)間過長(zhǎng),建議縮短顯色及曝光的時(shí)間。
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