WB、IHC、ELISA
Chicken
N/A
IgY
PBS, pH 7.4, 15mg/ml Bovine Serum Albumin as Stablizer and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
1:100-1:1,000 for immunohisto/cytochemistry
1:1,000-1:100,00 for ELLSA and Western blotting with chromogenic substrates
1:1,000-1:10,000 for Western blotting with ECL substates Optimal dilutions should be determined by the end user.
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Antibody Specificity:Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule pig IgG.
lt also reacts with the light chains of other pig immunoglobulins. No antibody was detected against non-immunoglobulin
serum proteins.The antibody may cross-react with immunoglobulins from other species.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable for
the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
更新中...
答：可能的原因有：
1、目的蛋白有多個修飾位點，本身可以呈現(xiàn)多條帶，建議查閱文獻或進行生物信息學(xué)分析，獲得蛋白序列的修飾位點信息，通過去修飾確定蛋白實際大小；
2、樣本處理過程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑；樣本處理時在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強，建議使用特異性強的抗體；
5、抗體孵育時間過久，建議減少抗體孵育時間；
6、二抗與抗原有交叉反應(yīng)，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質(zhì)變性過程及強度；
8、底物顯色與曝光時間過長，建議縮短顯色及曝光的時間。
Copyright ?湖北普美生物科技有限公司 All Rights Reserved. 工信部備案：鄂ICP備17001029號-1 技術(shù)支持：武漢微盟
