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GAPDH Mouse Monoclonal Antibody(2B8)-HRP conjugated
貨號:PMK053M/S
一鍵復制產品信息
價格:
680.00
規格:
50ul
100ul
貨期:
現貨
本產品4℃運輸,保存于4℃,保質期 12個月。
Details
產品詳情
  • Applications

    WB, IHC

  • Species Cross-Reactivity

    H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh

  • Molecular Weight

    37KD

  • Isotype

    IgG1

  • Storage Buffer & Condition

    PBS, pH 7.4, containing 0.02% Kathon  as Preservative and 50% Glycerol.Store at -20°C. 

    Do not aliquot the antibody.


  • Recommended dilutions

    WB: 1:1,0000-50000    

    IHC: 1:200


  • Specificity

    The GAPDH antibody can detects endogenous GAPDH protein.

  • Alternative Names

    G3PD, G3PDH, GAPD, Glyceraldehyde 3 phosphate dehydrogenase, MGC102544

  • Background

    Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. 

    GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as 

    loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH 

    expression in certain cell types.


Instruction manual/COA download
說明書/COA下載
Published literature
已發表文獻
  • 1.Hua-Zhen X ,Tong-Fei L ,Yan M , et al.Targeted photodynamic therapy of glioblastoma mediated by platelets with photo-controlled release property[J].Biomaterials,2022,290121833-121833. (IF 15.304)

    2.LiuGen L ,XiaoXin Y ,HuaZhen X , et al.A Dihydroartemisinin-Loaded Nanoreactor Motivates Anti-Cancer Immunotherapy by Synergy-Induced Ferroptosis to Activate Cgas/STING for Reprogramming of Macrophage.[J].Advanced healthcare materials,2023,12(28):e2301561-e2301561. (IF 10)

    3.Xin X Y ,Xiang X ,Fang M W , et al.A nanoreactor boosts chemodynamic therapy and ferroptosis for synergistic cancer therapy using molecular amplifier dihydroartemisinin[J].Journal of Nanobiotechnology,2022,20(1):230-230. (IF 9.429)

    4.Ning H ,Zi-Yi Y ,Zhong-Xiong X , et al.Dihydroartemisinin elicits immunogenic death through ferroptosis-triggered ER stress and DNA damage for lung cancer immunotherapy[J].Phytomedicine,2023,112154682-154682.  (IF 7.9)

    5.QiRui L ,HuaZhen X ,RongCheng X , et al.Platelets are highly efficient and efficacious carriers for tumor-targeted nano-drug delivery.[J].Drug delivery,2022,29(1):937-949.  (IF 6.819)

    6.Hu J ,Chen N N ,Li G L , et al.Cepharanthine-mediated endoplasmic reticulum stress inhibits Notch1 via binding GRP78 for suppressing hepatocellular carcinoma metastasis[J].Phytomedicine,2024,135156162-156162. ( IF 6.7)

    7.Li T ,Xu Y ,Li K , et al.Doxorubicin-polyglycerol-nanodiamond composites stimulate glioblastoma cell immunogenicity through activation of autophagy[J].Acta Biomaterialia,2019,86381-394. (IF 6.638)

    8.Xiao Y C ,Tang Y ,Ren T , et al.Treatment of silicosis with quercetin depolarizing macrophages via inhibition of mitochondrial damage-associated pyroptosis[J].Ecotoxicology and Environmental Safety,2024,286117161-117161. (IF 6.2)

    9. Li G L ,Hu J ,Han N , et al.Dihydroartemisinin-driven TOM70 inhibition leads to mitochondrial destabilization to induce pyroptosis against lung cancer.[J].Phytotherapy research : PTR,2024,38(8):3856-3876.  (6.1)

    10. Meiyao W ,Huazhen X ,Tongfei L , et al.Sonodynamic therapy of glioblastoma mediated by platelets with ultrasound-triggered drug release.[J].Drug delivery,2023,30(1):2219429-2219429.  (IF 6)

    11.Ting T Y ,Ning H ,Gen L L , et al.Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING[J].Frontiers in Pharmacology,2022,13837784-837784.  (IF 5.988)

    12. Gen L L ,Chun X P ,Ting T Y , et al.Dihydroartemisinin remodels macrophage into an M1 phenotype via ferroptosis-mediated DNA damage[J].Frontiers in Pharmacology,2022,13949835-949835.  (IF 5.988)

    13. Xu Z H ,Chen X F ,Li K , et al.Anti-lung cancer synergy of low-dose doxorubicin and PD-L1 blocker co-delivered via mild photothermia-responsive black phosphorus.[J].Drug delivery and translational research,2024,15(1):1-22. (IF 5.7)

    14.Ting-Ting Y ,Jun H ,Qi-Rui L , et al.Chlorin e6-induced photodynamic effect facilitates immunogenic cell death of lung cancer as a result of oxidative endoplasmic reticulum stress and DNA damage[J].International Immunopharmacology,2023,115. (IF 5.6)

    15.Ning H ,LiuGen L ,XingChun P , et al.Ferroptosis triggered by dihydroartemisinin facilitates chlorin e6 induced photodynamic therapy by inhibiting GPX4 and enhancing ROS.[J].European journal of pharmacology,2022,919(prepublish):174797-174797. (IF 5.195)

    16.Bai F X ,Hu J ,Wang F M , et al.Cepharanthine Triggers Ferroptosis Through Inhibition of NRF2 For Robust ER Stress Against Lung Cancer.[J].European journal of pharmacology,2024,979176839. (IF 4.2)

    17.Jian Y H ,Ting T Y ,Gen L L , et al.B-Myb deficiency boosts bortezomib-induced immunogenic cell death in colorectal cancer[J].Scientific Reports,2024,14(1):7733-7733. (IF 3.8)

    18.Gen L L ,Chun X P ,Yi Z Y , et al.Dihydroartemisinin-driven selective anti-lung cancer proliferation by binding to EGFR and inhibition of NRAS signaling pathway-induced DNA damage[J].Scientific Reports,2024,14(1):11704-11704. (IF 3.8)

    19. YuanJian H ,Hao C ,XingChun P , et al.Up-regulation of ABCG2 by MYBL2 deletion drives Chlorin e6-mediated photodynamic therapy resistance in colorectal cancer.[J].Photodiagnosis and photodynamic therapy,2023,42103558-103558.  (IF 3.3)

    20. Li G L ,Zhang D ,Huang Q , et al.Mitochondrial disruption resulting from Cepharanthine-mediated TOM inhibition triggers ferroptosis in colorectal cancer cells[J].Journal of Cancer Research and Clinical Oncology,2024,150(10):460-460. (IF 2.7)

    21.Gufeng L ,Quan S ,Xiaoyi W , et al.Clock genes are expressed in cementum and regulate the proliferation and mineralization of cementoblasts.[J].In vitro cellular & developmental biology. Animal,2023,59(1):76-84.(IF 2.1)
    22.C. L ,J. Y ,B. L , et al.BACH1 regulates the proliferation and odontoblastic differentiation of human dental pulp stem cells[J].BMC Oral Health,2022,22(1):536-536.
    (IF 3.747)
    23.Minmin S ,Yaoting J ,Shuhui Z , et al.Ginsenoside Rb3 inhibits osteoclastogenesis via ERK/NF-κB signaling pathway in vitro and in vivo.[J].Oral diseases,2022,29(8):3460-3471.
    (IF 4.068)
    24.Tingting L ,Xiazhou F ,Yaoli L , et al.Sulforaphane Inhibits Osteoclastogenesis via Suppression of the Autophagic Pathway[J].Molecules,2021,26(2):347-347.
    (IF 4.412)
    25.Xiaoyu L ,Beike W ,Maolin C , et al.USP12 regulates ER stress-associated osteogenesis in human periodontal ligament cells under tension stress[J].Cellular Signalling,2024,114111015-.
    (IF 4.8)
    26.Gufeng L ,Guixin Z ,Xiaoyi W , et al.Thy-1 knockdown promotes the osteogenic differentiation of GMSCs via the Wnt/β-catenin pathway.[J].Journal of cellular and molecular medicine,2023,27(23):3805-3815.
    (IF 5.3)
    27.Ding F ,Zheng P ,Yan Y X , et al.Adipocyte-secreted PRELP promotes adipocyte differentiation and adipose tissue fibrosis by binding with p75NTR to activate FAK/MAPK signaling.[J].International journal of biological macromolecules,2024,279(P4):135376.
    (IF 7.7)

Product Q&A FAQ
產品問答FAQ
  • Western Blot(WB)實驗中有很多雜帶咋回事?

    答:可能的原因有:

    1、目的蛋白有多個修飾位點,本身可以呈現多條帶,建議查閱文獻或進行生物信息學分析,獲得蛋白序列的修飾位點信息,通過去修飾確定蛋白實際大??;

    2、樣本處理過程中目的蛋白發生降解,建議加入蛋白酶抑制劑;樣本處理時在冰上操作;

    3、雜蛋白多,建議處理目的蛋白;

    4、抗體特異性不強,建議使用特異性強的抗體;

    5、抗體孵育時間過久,建議減少抗體孵育時間;

    6、二抗與抗原有交叉反應,建議選擇合適的封閉物;

    7、二聚體或多聚體存在,建議增加蛋白質變性過程及強度;

    8、底物顯色與曝光時間過長,建議縮短顯色及曝光的時間。


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