WB
H, R, M
42KD
IgG
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
WB: 1:2,000-5,000
The α-Smooth Muscle Actin antibody can detects endogenous α-Smooth Muscle Actin protein.
AAT6 antibody, ACTA2 antibody, alpha actin 2 antibody, GIG46 antibody, ACTSA antibody, ACTVS antibody
Actin is a globular multi-functional protein that forms microfilaments. ACTA2 codes for an α-actin located
in the smooth muscle, and also in vascular smooth muscle.
更新中...
答:可能的原因有:
1、目的蛋白有多個(gè)修飾位點(diǎn),本身可以呈現(xiàn)多條帶,建議查閱文獻(xiàn)或進(jìn)行生物信息學(xué)分析,獲得蛋白序列的修飾位點(diǎn)信息,通過去修飾確定蛋白實(shí)際大小;
2、樣本處理過程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑;樣本處理時(shí)在冰上操作;
3、雜蛋白多,建議處理目的蛋白;
4、抗體特異性不強(qiáng),建議使用特異性強(qiáng)的抗體;
5、抗體孵育時(shí)間過久,建議減少抗體孵育時(shí)間;
6、二抗與抗原有交叉反應(yīng),建議選擇合適的封閉物;
7、二聚體或多聚體存在,建議增加蛋白質(zhì)變性過程及強(qiáng)度;
8、底物顯色與曝光時(shí)間過長,建議縮短顯色及曝光的時(shí)間。
Copyright ?湖北普美生物科技有限公司 All Rights Reserved. 工信部備案:鄂ICP備17001029號(hào)-1 技術(shù)支持:武漢微盟