WB, IP
H, R, M
68KD
IgG1
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20 °C.
Do not aliquot the antibody.
WB: 1:1,0 00-3,000
IHC: 1:500-1,000
The Lamin B1 antibody ca n detects endogenous Lamin B1 protein.
ADLD, LMB1,LMNB1 , MGC111419, OTTHUMP00000159218
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane.
The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina
matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be
involved in nuclear stability, chromatin structure and gene expression. Vertebrate la mins consist of two types, A
and B. This gene encodes one of the two B type proteins, B1.
更新中...
答：可能的原因有：
1、目的蛋白有多個(gè)修飾位點(diǎn)，本身可以呈現(xiàn)多條帶，建議查閱文獻(xiàn)或進(jìn)行生物信息學(xué)分析，獲得蛋白序列的修飾位點(diǎn)信息，通過去修飾確定蛋白實(shí)際大?。?/p>
2、樣本處理過程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑；樣本處理時(shí)在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強(qiáng)，建議使用特異性強(qiáng)的抗體；
5、抗體孵育時(shí)間過久，建議減少抗體孵育時(shí)間；
6、二抗與抗原有交叉反應(yīng)，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質(zhì)變性過程及強(qiáng)度；
8、底物顯色與曝光時(shí)間過長，建議縮短顯色及曝光的時(shí)間。
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