WB, IHC, IF
H, R, M, Mk, Ch, Hm, Sh,Xenopus
43KD
IgG1
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
WB: 1:3,000
IHC: 1:200
IF: 1:200
Antibody can detects endogenous β-actin protein.
β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and
tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful
as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells.
For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control
for these tissues.
1.MingJun S ,PingPing T ,ZhongQiang L , et al.MicroRNA-27a targets Sfrp1 to induce renal fibrosis in diabetic nephropathy by activating Wnt/β-Catenin signalling.[J].Bioscience reports,2020,40(6):(IF 2.942)
2.Liu L ,Wang Y ,Yan R , et al.BMP-7 inhibits renal fibrosis in diabetic nephropathy via miR-21 downregulation[J].Life Sciences,2019,238(C):116957.(IF 3.448)
3.Liyuan H ,Hualing S ,Fangfang S , et al.SIRT6 overexpression inhibits cementogenesis by suppressing glucose transporter 1.[J].Journal of cellular physiology,2019,234(4):4005-4014.(IF 3.923)
4.Xin S ,Shengyu W ,Xian L , et al.Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells.[J].Proteomics,2017,17(12):n/a-n/a.(IF 4.041)
5.Liyuan H ,Fangfang S ,Hualing S , et al.IRX5 promotes NF-κB signalling to increase proliferation, migration and invasion via OPN in tongue squamous cell carcinoma.[J].Journal of cellular and molecular medicine,2018,22(8):3899-3910.(IF 4.302)
6.Yuanyuan W ,Lingling L ,Wei P , et al.Ski-related novel protein suppresses the development of diabetic nephropathy by modulating transforming growth factor-β signaling and microRNA-21 expression.[J].Journal of cellular physiology,2019,234(10):17925-17936.(IF 4.522)
7.Wenqi L ,Shiwen K ,Huijian C , et al.A composition of ursolic acid derivatives from Ludwigia hyssopifolia induces apoptosis in throat cancer cells via the Akt/mTOR and mitochondrial signaling pathways and by modulating endoplasmic reticulum stress.[J].Journal of ethnopharmacology,2023,319(P3):117351-117351.(IF 5.4)
8.Huiming L ,Rui Y ,Luqun L , et al.The role of CDX2 in renal tubular lesions during diabetic kidney disease.[J].Aging,2021,13(5):(IF 5.682)
9.Jiayi X ,Huifang Z ,Xingcheng Z , et al.Atorvastatin Restores PPARα Inhibition of Lipid Metabolism Disorders by Downregulating miR-21 Expression to Improve Mitochondrial Function and Alleviate Diabetic Nephropathy Progression[J].Frontiers in Pharmacology,2022,13819787-819787.(IF 5.988)
10.Liyuan H ,Jie S ,Lingqing D , et al.Simultaneous acceleration of osteogenesis and angiogenesis by surface oxygen vacancies of rutile nanorods[J].Colloids and Surfaces B: Biointerfaces,2022,212(prepublish):112348-.(IF 5.999)
11.Yerlan B ,Wenqi L ,Muguli M , et al.Ferula ferulaeoides ethyl acetate extract induces apoptosis in esophageal cancer cells via mitochondrial and PI3K/Akt/Bad pathways[J].Arabian Journal of Chemistry,2023,16(12):(IF 6)
12.Qian Z ,Zhen H ,Huanyan Z , et al.Chromatin Accessibility Predetermines Odontoblast Terminal Differentiation.[J].Frontiers in cell and developmental biology,2021,9769193-769193.(IF 6.684)
13.HuiFang Z ,HuiMing L ,JiaYi X , et al.Alpha lipoamide inhibits diabetic kidney fibrosis via improving mitochondrial function and regulating RXRα expression and activation.[J].Acta pharmacologica Sinica,2022,44(5):1051-1065.(IF 7.169)
答：可能的原因有：
1、目的蛋白有多個修飾位點，本身可以呈現多條帶，建議查閱文獻或進行生物信息學分析，獲得蛋白序列的修飾位點信息，通過去修飾確定蛋白實際大小；
2、樣本處理過程中目的蛋白發生降解,建議加入蛋白酶抑制劑；樣本處理時在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強，建議使用特異性強的抗體；
5、抗體孵育時間過久，建議減少抗體孵育時間；
6、二抗與抗原有交叉反應，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質變性過程及強度；
8、底物顯色與曝光時間過長，建議縮短顯色及曝光的時間。
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