WB, IHC, IF
H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh
43KD
IgG1
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
WB: 1:5,000
IHC: 1:200
IF: 1:200
Antibody can detects endogenous β-actin protein.
β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species
and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are
useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain
cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading
control for these tissues.
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答：可能的原因有：
1、目的蛋白有多個修飾位點，本身可以呈現多條帶，建議查閱文獻或進行生物信息學分析，獲得蛋白序列的修飾位點信息，通過去修飾確定蛋白實際大小；
2、樣本處理過程中目的蛋白發生降解,建議加入蛋白酶抑制劑；樣本處理時在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強，建議使用特異性強的抗體；
5、抗體孵育時間過久，建議減少抗體孵育時間；
6、二抗與抗原有交叉反應，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質變性過程及強度；
8、底物顯色與曝光時間過長，建議縮短顯色及曝光的時間。
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