WB, IP
H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh
43KD
IgG1
PBS, pH 7.4, containing 0.02% Kathon as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody
WB: 1:5000
IHC: 1:100-500
Antibodycandetectsendogenous β-Actin protein.
actin antibody, b-actin antibody, beta-actin antibody
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and
tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful
as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells.
For example, expression of β-actin in adiposetissue is very low and therefore β-actin should not be used as loading control for
these tissues.
1. Yao X ,Shengbo J ,Miao H , et al.Chromatin conformation of human oral epithelium can identify orofacial cleft missing functional variants[J].International Journal of Oral Science,2022,14(1):43-43. (IF 24.897)
2. Shiyu L ,Qi Z ,Songhang L , et al.Tetrahedral framework nucleic acids‐based delivery promotes intracellular transfer of healing peptides and accelerates diabetic would healing[J].Cell Proliferation,2022,55(9):e13279-e13279. (IF 8.755)
3. Konghuai W ,Lu Z ,Hanqing M , et al.Intercellular mitochondrial transfer alleviates pyroptosis in dental pulp damage.[J].Cell proliferation,2023,56(9):e13442-e13442. (IF 8.5)
4. Hui L ,Yuxuan L ,Wei F , et al.Fusobacterium nucleatum triggers proinflammatory cell death via Z-DNA binding protein 1 in apical periodontitis[J].Cell Communication and Signaling,2022,20(1):196-196. (IF 7.525)
5. Sun Y ,Chen Y ,Zhao H , et al.Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma[J].iScience,2024,27(4):109340-. (IF 4.6)
6. Huang D ,Li Y ,Han J , et al.Xbp1 promotes odontoblastic differentiation through modulating mitochondrial homeostasis.[J].FASEB journal : official publication of the Federation of American Societies for Experimental Biology,2024,38(7):e23600-e23600. (IF 4.4)
7. Huanyan Z ,Yao X ,Jiahao H , et al.Phosphorylation of ATF2 promotes odontoblastic differentiation via intrinsic HAT activity.[J].Journal of genetics and genomics = Yi chuan xue bao,2023,50(7):497-510. (IF 5.9)
8. Xin W ,MingJing H ,XiaoJie C , et al.Glaucocalyxin A impairs tumor growth via amplification of the ATF4/CHOP/CHAC1 cascade in human oral squamous cell carcinoma.[J].Journal of ethnopharmacology,2022,290115100-115100. (IF 5.195)
9. Zhou L ,Shi W ,Zhang X , et al.Injectable Tannin-Containing Hydroxypropyl Chitin Hydrogel as Novel Bioactive Pulp Capping Material Accelerates Repair of Inflamed Dental Pulp.[J].Biomolecules,2024,14(9):1129-1129. (IF 4.8)
10. Liu H ,Liu X Y ,Fan W , et al.Metformin switches cell death modes to soothe the apical periodontitis via ZBP1.[J].FASEB journal : official publication of the Federation of American Societies for Experimental Biology,2024,38(5):e23549-e23549. (IF 4.4)
11. Min C ,Siyang Z ,Siyu C , et al.Pharmacological inhibition of SMYD2 protects against cisplatin-induced renal fibrosis and inflammation[J].Journal of Pharmacological Sciences,2023,153(1):38-45. (IF 3.5)
12. Tian-Qi M ,Nan-Nan C ,Rong-Cheng X , et al.Indocyanine green-loaded platelet activated by photodynamic and photothermal effects for selective control of wound repair[J].Photodiagnosis and Photodynamic Therapy,2024,45103945-. (IF 3.3)
Gao Y ,Lin Y ,Li Y , et al.Interplay of RUNX2 and KLF4 in initial commitment of odontoblast differentiation.[J].Journal of cellular biochemistry,2024,125(7): (IF 3)
答：可能的原因有：
1、目的蛋白有多個修飾位點，本身可以呈現(xiàn)多條帶，建議查閱文獻或進行生物信息學(xué)分析，獲得蛋白序列的修飾位點信息，通過去修飾確定蛋白實際大小；
2、樣本處理過程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑；樣本處理時在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強，建議使用特異性強的抗體；
5、抗體孵育時間過久，建議減少抗體孵育時間；
6、二抗與抗原有交叉反應(yīng)，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質(zhì)變性過程及強度；
8、底物顯色與曝光時間過長，建議縮短顯色及曝光的時間。
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